ESPU Congress 2018 - Abstract Book

11–14 APRIL, 2018, HELSINKI, FINLAND

13:45–13:48

S1-6 (PP)

MICRORNA-132 IN BLADDER WOUND HEALING

Xi LIU

, Clara Ibel CHAMORRO

, Dongqing LI

, Ning XU LANDÉN

Behnaz Khalilzadeh BINICY

and Magdalena FOSSUM

1) Karolinska Institutet, Women's and Children's Health, Stockholm, SWEDEN - 2) Karolinska Institutet, Medicine,

Stockholm, SWEDEN - 3) Örebro Universitet, Medicine, örebro, SWEDEN - 4) Karolinska Institutet/Karolinska Hospital,

Women's and Children's Health/Patient Area for Children with urogenital malformation, Stockholm, SWEDEN

PURPOSE

In regenerative urogenital medicine, we hypothesize that a deeper understanding of normal bladder

wound healing could further enlighten our understanding of essential factors related to regenerative

medicine in healthy and diseased tissues.

MicroRNAs (miRs) are a group of conservative small non-coding RNAs, crucial for post-transcrip-

tional regulation in most biological events including wound healing.

In skin, miR-132 has been reported as a key regulator for keratinocyte migration, proliferation and

inflammation.

The aim of this study was to analyse if miR-132 could be of importance to enhance urothelial cell

migration and proliferation for wound closure.

MATERIAL AND METHODS

Human urothelial cells were isolated from bladder washings or biopsies. Cells were cultured until

confluence before performing a standardized 2D scratch assay. The expression of miR-132 was

analysed upon wounding after 6 h, 12 h, 24 h using real-time quantitative PCR by the – delta delta

Ct with an internal control for U6 and a non-scratch control group (6 replicates per group).

RESULTS

The area of wound gap was significantly reduced within 24 h, miR-132 was up-regulated 1,8 times

6 h after wounding. At 12 h and 24 h after wounding, miR-132 was up regulated 1,4 times. Same

results were found in primary urothelial cells isolated from bladder washes as from bladder biopsies.

CONCLUSIONS

Our results indicate that miR-132 expression was induced after wounding, and demonstrated

a similar expression pattern as in normal skin wound healing. miR-132 could be an important factor

for promoting urinary bladder wound healing, most probably by enhancing urothelial migration and

suggests us to proceed with validations including functional studies.