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CONGRESS OF THE ESPU
13:48–13:51
S1-7 (PP)
URETRAL REPLACEMENT USING TUBULARIZED
COLLAGEN SCAFFOLD IMPLEMENTED WITH ADIPOSE
DERIVED STROMAL CELLS
Melodie JURICIC
1
, Kalitha PINNAGODA
2
, Marion BOURDENS
3
, Nicolas GAIDE
4
,
Elisabeth JEUNESSE
4
, Matthias HANS LARSON
5
, Isabelle RAYMOND-LETRON
4
,
Valerie PLANAT
3
and Olivier ABBO
1
1) Hôpital des Enfants - CHU Toulouse, Pediatric Surgery Department, Toulouse, FRANCE - 2) CHUV Lausanne -EPFL,
Pediatric surgery -DMPC, Lausanne, SWITZERLAND - 3) Stromalab - UMR 1031 - Inserm - CNRS, Toulouse, FRANCE
- 4) ENVT, Toulouse, FRANCE - 5) EPFL, Lausanne, SWITZERLAND
PURPOSE
Tissue engineering has emerged as a promising alternative approach in uretral reconstruction.
Pinnagoda and al (Acta Biomat 2016) have successfully implanted an acellular double-layered
collagen scaffolds as grafts for uretral replacement in a rabbit model.
The value of cell-seeding scaffold to repair uretral defects has been underlined by several studies.
Among the potential useful, Adipose derived stem cells (ADSCs) are well described, with differen-
ciation, proangiogenic and immunomodulative properties.
Therefore the aim of our study was firstly to seed ADSC into the previously described tubular col-
lagen scaffold in order to analyze the in vitro features of the cells into the scaffold. Secondly we
aimed to determine the role of the cells concerning the scaffold integration in a rabbit model of
urethral defect.
MATERIAL AND METHODS
ADSCs were isolated from rabbit adipose tissue, then implemented during the polymerisation of the
collagen tubularized scaffold. In vitro analysis concerned cellular morphology, viabily, proliferation
and gene expression profile.
In 10 New Zealand Rabbits, 2 cm long grafts were sutured to replace subtotal excision of uretra.
They were prospectively randomised in 2 groups of 5 rabbits depending on the presence of seeded
ADSC into the collagen scaffold.
Histological and clinical results were compared after 10 days.
RESULTS
ADSCs within collagen scaffold retains characteristic morphology, are viable, proliferate, and have
a gene expression profile comparable to ASCs growing in 2D culture.
In vivo, clinical and histological evaluation 10 days after uretral reconstruction demonstrated integra-
tion and early epithelialization in both groups. An increase inflammation appareance was observed
in the « ASCs group » where the cells were still present and healthy in the implented group.
CONCLUSIONS
Tubiular Collagen scaffold seeded with ADSCs is feasible and may offer a useful alternative in the
future for patients requiring tissular replacement. Further studies with longer.
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Discussion